Silencing nuclear pore protein Tpr elicits a senescent-like phenotype in cancer cells.

ABSTRACT:

Background

Tpr is a large coiled-coil protein located in the nuclear basket of the nuclear pore complex for which many different functions were proposed from yeast to human.

Methodology/principal findings

Here we show that depletion of Tpr by RNA interference triggers G0-G1 arrest and ultimately induces a senescent-like phenotype dependent on the presence of p53. We also found that Tpr depletion impairs the NES [nuclear export sequence]-dependent nuclear export of proteins and causes partial co-depletion of Nup153. In addition Tpr depletion impacts on level and function of the SUMO-protease SENP2 thus affecting SUMOylation regulation at the nuclear pore and overall SUMOylation in the cell.

Conclusions

Our data for the first time provide evidence that a nuclear pore co

SUBMITTER: David-Watine B 

PROVIDER: S-EPMC3139644 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature