Ontology highlight
ABSTRACT:
SUBMITTER: Craig TA
PROVIDER: S-EPMC3145175 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Craig Theodore A TA Sommer Stacy L SL Beito Thomas G TG Kumar Rajiv R
Hybridoma (2005) 20091001 5
We developed and characterized monoclonal antibodies directed against the amino-terminal and carboxy-terminal regions of human and mouse sclerostin (scl). Amino-terminal and carboxy-terminal scl peptides with limited homology to scl domain-containing protein-1 were synthesized using f-moc chemistry. The peptides were conjugated to keyhole limpet hemocyanin and the conjugates were used for immunization of mice. Monoclonal antibodies were obtained and characterized using bacterially expressed and ...[more]