Ontology highlight
ABSTRACT:
SUBMITTER: Wu R
PROVIDER: S-EPMC3146562 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Wu Ronghu R Haas Wilhelm W Dephoure Noah N Huttlin Edward L EL Zhai Bo B Sowa Mathew E ME Gygi Steven P SP
Nature methods 20110703 8
The functional role of protein phosphorylation is impacted by its fractional stoichiometry. Thus, a comprehensive strategy to study phosphorylation dynamics should include an assessment of site stoichiometry. Here we report an integrated method that relies on phosphatase treatment and stable-isotope labeling to determine absolute stoichiometries of protein phosphorylation on a large scale. This approach requires the measurement of only a single ratio relating phosphatase-treated and mock-treated ...[more]