Identification of epigenetic DNA modifications with a protein nanopore.
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ABSTRACT:
SUBMITTER: Wallace EV
PROVIDER: S-EPMC3147113 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Identification of epigenetic DNA modifications with a protein nanopore.
Wallace Emma V B EV Stoddart David D Heron Andrew J AJ Mikhailova Ellina E Maglia Giovanni G Donohoe Timothy J TJ Bayley Hagan H
Chemical communications (Cambridge, England) 20101006 43
Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA. ...[more]
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