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Protein identification by nanopore peptide profiling.

ABSTRACT: Nanopores are single-molecule sensors used in nucleic acid analysis, whereas their applicability towards full protein identification has yet to be demonstrated. Here, we show that an engineered Fragaceatoxin C nanopore is capable of identifying individual proteins by measuring peptide spectra that are produced from hydrolyzed proteins. Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles, hence allowing protein fingerprinting. The intensity of individual peaks provides information on the concentration of individual peptides, indicating that this approach is quantitative. Our work shows the potential of a low-cost, portable nanopore-based analyzer for protein identification.

SUBMITTER: Lucas FLR

PROVIDER: S-EPMC8490355 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Protein identification by nanopore peptide profiling.

Lucas Florian Leonardus Rudolfus FLR Versloot Roderick Corstiaan Abraham RCA Yakovlieva Liubov L Walvoort Marthe T C MTC Maglia Giovanni G

Nature communications 20211004 1

Nanopores are single-molecule sensors used in nucleic acid analysis, whereas their applicability towards full protein identification has yet to be demonstrated. Here, we show that an engineered Fragaceatoxin C nanopore is capable of identifying individual proteins by measuring peptide spectra that are produced from hydrolyzed proteins. Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles, hence allowing protein fingerprin ...[more]

PMID: 34608150

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