Ontology highlight
ABSTRACT:
SUBMITTER: Lucas FLR
PROVIDER: S-EPMC8490355 | biostudies-literature | 2021 Oct
REPOSITORIES: biostudies-literature
Lucas Florian Leonardus Rudolfus FLR Versloot Roderick Corstiaan Abraham RCA Yakovlieva Liubov L Walvoort Marthe T C MTC Maglia Giovanni G
Nature communications 20211004 1
Nanopores are single-molecule sensors used in nucleic acid analysis, whereas their applicability towards full protein identification has yet to be demonstrated. Here, we show that an engineered Fragaceatoxin C nanopore is capable of identifying individual proteins by measuring peptide spectra that are produced from hydrolyzed proteins. Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles, hence allowing protein fingerprin ...[more]