Ontology highlight
ABSTRACT:
SUBMITTER: Perez C
PROVIDER: S-EPMC3147255 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Perez Camilo C Khafizov Kamil K Forrest Lucy R LR Krämer Reinhard R Ziegler Christine C
EMBO reports 20110617 8
The osmoregulated betaine transporter BetP is a stable trimer. Structural studies have shown that individual protomers can adopt distinct transport conformations, implying a functional role for the trimeric state in transport, although the role of trimerization in regulation is not yet understood. We designed putative monomeric mutants by molecular-dynamics simulations and in silico alanine-scanning mutagenesis. Several mutants including BetP-W101A/T351A were monomeric in detergent as well as in ...[more]