Ontology highlight
ABSTRACT:
SUBMITTER: Miracco EJ
PROVIDER: S-EPMC3150545 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20110715 31
The pseudouridine synthase TruB handles 5-fluorouridine in RNA as a substrate, converting it into two isomeric hydrated products. Unexpectedly, the two products differ not in the hydrated pyrimidine ring but in the pentose ring, which is epimerized to arabinose in the minor product. This inversion of stereochemistry at C2' suggests that pseudouridine generation may proceed by a mechanism involving a glycal intermediate or that the previously proposed mechanism involving an acylal intermediate op ...[more]