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Purification, crystallization and preliminary X-ray crystallographic study of the tRNA pseudouridine synthase TruB from Streptococcus pneumoniae.

ABSTRACT: The RNA pseudouridine synthase TruB catalyses the isomerization of uridine to pseudouridine (?) at residue 55 of elongator tRNAs. In order to better elucidate the functions of TruB in the formation of pseudouridine, the three-dimensional structure of full-length TruB was determined by X-ray crystallography. Here, the expression, purification, crystallization and preliminary crystallographic analysis of TruB from Streptococcus pneumoniae are reported. The crystal belonged to space group P2, with unit-cell parameters a = 37.65, b = 78.09, c = 56.33 Å, ? = 102.05°, and diffracted to a resolution of 1.7 Å. The crystal is most likely to contain one molecule in the asymmetric unit, with a VM value of 2.40 Å(3) Da(-1).

SUBMITTER: Yang W

PROVIDER: S-EPMC3702319 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Purification, crystallization and preliminary X-ray crystallographic study of the tRNA pseudouridine synthase TruB from Streptococcus pneumoniae.

Yang Wei W Zhao Shasha S Jin Li L Guo Zhen Z Zhang Shaocheng S Zhang Hongpeng H Wang Deqiang D

Acta crystallographica. Section F, Structural biology and crystallization communications 20130628 Pt 7

The RNA pseudouridine synthase TruB catalyses the isomerization of uridine to pseudouridine (Ψ) at residue 55 of elongator tRNAs. In order to better elucidate the functions of TruB in the formation of pseudouridine, the three-dimensional structure of full-length TruB was determined by X-ray crystallography. Here, the expression, purification, crystallization and preliminary crystallographic analysis of TruB from Streptococcus pneumoniae are reported. The crystal belonged to space group P2, with ...[more]

PMID: 23832202

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