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Near-atomic-resolution cryo-EM for molecular virology.

ABSTRACT: Electron cryo-microscopy (cryo-EM) is a technique in structural biology that is widely used to solve the three-dimensional structures of macromolecular assemblies, close to their biological and solution conditions. Recent improvements in cryo-EM and single-particle reconstruction methodologies have led to the determination of several virus structures at near-atomic resolution (3.3 - 4.6 Å). These cryo-EM structures not only resolve the C? backbones and side-chain densities of viral capsid proteins, but also suggest functional roles that the protein domains and some key amino acid residues play. This paper reviews the recent advances in near-atomic-resolution cryo-EM for probing the mechanisms of virus assembly and morphogenesis.

SUBMITTER: Hryc CF

PROVIDER: S-EPMC3155204 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Near-atomic-resolution cryo-EM for molecular virology.

Hryc Corey F CF Chen Dong-Hua DH Chiu Wah W

Current opinion in virology 20110801 2

Electron cryo-microscopy (cryo-EM) is a technique in structural biology that is widely used to solve the three-dimensional structures of macromolecular assemblies, close to their biological and solution conditions. Recent improvements in cryo-EM and single-particle reconstruction methodologies have led to the determination of several virus structures at near-atomic resolution (3.3 - 4.6 Å). These cryo-EM structures not only resolve the Cα backbones and side-chain densities of viral capsid protei ...[more]

PMID: 21845206

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