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De novo protein structure determination from near-atomic-resolution cryo-EM maps.

ABSTRACT: We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps.

SUBMITTER: Wang RY

PROVIDER: S-EPMC4435692 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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De novo protein structure determination from near-atomic-resolution cryo-EM maps.

Wang Ray Yu-Ruei RY Kudryashev Mikhail M Li Xueming X Egelman Edward H EH Basler Marek M Cheng Yifan Y Baker David D DiMaio Frank F

Nature methods 20150223 4

We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron micros ...[more]

PMID: 25707029

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