Unknown

Dataset Information

0

The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines.


ABSTRACT: Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, in vitro methylation of the BART and Cp promoters reduced the promoter-driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.

SUBMITTER: Kim DN 

PROVIDER: S-EPMC3158499 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines.

Kim Do Nyun DN   Song Yoon Jae YJ   Lee Suk Kyeong SK  

Experimental & molecular medicine 20110701 7


Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became  ...[more]

Similar Datasets

| S-EPMC3393554 | biostudies-literature
| S-EPMC387356 | biostudies-literature
2023-02-27 | MSV000091375 | MassIVE
| S-EPMC7189298 | biostudies-literature
| S-EPMC5774230 | biostudies-literature
| S-EPMC7062761 | biostudies-literature
| S-EPMC4820504 | biostudies-literature
| S-EPMC2715772 | biostudies-literature
| S-EPMC8206016 | biostudies-literature
| S-EPMC6146817 | biostudies-literature