Quantification of mRNA and protein and integration with protein turnover in a bacterium.

ABSTRACT: Biological function and cellular responses to environmental perturbations are regulated by a complex interplay of DNA, RNA, proteins and metabolites inside cells. To understand these central processes in living systems at the molecular level, we integrated experimentally determined abundance data for mRNA, proteins, as well as individual protein half-lives from the genome-reduced bacterium Mycoplasma pneumoniae. We provide a fine-grained, quantitative analysis of basic intracellular processes under various external conditions. Proteome composition changes in response to cellular perturbations reveal specific stress response strategies. The regulation of gene expression is largely decoupled from protein dynamics and translation efficiency has a higher regulatory impact on protein abundance

SUBMITTER: Maier T 

PROVIDER: S-EPMC3159969 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature