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ABSTRACT:
SUBMITTER: Arkhipov A
PROVIDER: S-EPMC3160275 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Arkhipov Anton A Schulten Klaus K
Optics express 20090201 4
Employing interference patterns for illumination has been shown to reduce the focal volume in fluorescence microscopy. For example, the 4Pi technique employs two interfering laser beams and significantly decreases the focal volume, as compared to conventional microscopy. We study theoretically the effect of using multiple interfering laser beams on the focal volume. In realistic setups with three or four beams, the focal volume is about half of that from the 4Pi case. This improvement reaches a ...[more]