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Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy.

ABSTRACT: An ideal illumination for light sheet fluorescence microscopy entails both a localized and a propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions, but their non-diffracting feature comes at the cost of the presence of high-energy side lobes that notably degrade the imaging contrast and induce photobleaching. Here, we demonstrate the use of a light droplet illumination whose side lobes are suppressed by interfering Bessel beams of specific k-vectors. Our droplet illumination readily achieves more than 50% extinction of the light distributed across the Bessel side lobes, providing a more efficient energy localization without loss in transverse resolution. In a standard light sheet fluorescence microscope, we demonstrate a two-fold contrast enhancement imaging micron-scale fluorescent beads. Results pave the way to new opportunities for rapid and deep in vivo observations of large-scale biological systems.

SUBMITTER: Di Domenico G

PROVIDER: S-EPMC6249239 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy.

Di Domenico Giuseppe G Ruocco Giancarlo G Colosi Cristina C DelRe Eugenio E Antonacci Giuseppe G

Scientific reports 20181121 1

An ideal illumination for light sheet fluorescence microscopy entails both a localized and a propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions, but their non-diffracting feature comes at the cost of the presence of high-energy side lobes that notably degrade the imaging contrast and induce photobleaching. Here, we demonstrate the use of a light droplet illumination whose side lobes are suppressed by interfering Bessel beams of specific k-vectors. Our dropl ...[more]

PMID: 30464219

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