Unknown

Dataset Information

0

An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells.

ABSTRACT: A major challenge in stem cell-mediated regenerative medicine is the development of defined culture systems for the maintenance of clinical-grade human embryonic stem (hES) cells. Here, we identify, using a feedback system control scheme, a unique combination of three small molecule inhibitors that enables the maintenance of hES cells on a fibronectin-coated surface through single cell passaging. After 20 passages, the undifferentiated state of the hES cells was confirmed by OCT4, SSEA4 and NANOG expressions, whereas their pluripotent potential and genetic integrity were demonstrated by teratoma formation and normal karyotype, respectively. Our study attests to the power of the feedback system control scheme to quickly pinpoint optimal conditions for desired biological activities, and provides a chemically defined, scalable and single cell passaging culture system for hES cells.

SUBMITTER: Tsutsui H 

PROVIDER: S-EPMC3161511 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells.

Tsutsui Hideaki H   Valamehr Bahram B   Hindoyan Antreas A   Qiao Rong R   Ding Xianting X   Guo Shuling S   Witte Owen N ON   Liu Xin X   Ho Chih-Ming CM   Wu Hong H  

Nature communications 20110101

A major challenge in stem cell-mediated regenerative medicine is the development of defined culture systems for the maintenance of clinical-grade human embryonic stem (hES) cells. Here, we identify, using a feedback system control scheme, a unique combination of three small molecule inhibitors that enables the maintenance of hES cells on a fibronectin-coated surface through single cell passaging. After 20 passages, the undifferentiated state of the hES cells was confirmed by OCT4, SSEA4 and NANO  ...[more]

Similar Datasets

Unknown Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors.
| S-EPMC3100988 | biostudies-literature
Unknown Optimized methods for extracting circulating small RNAs from long-term stored equine samples.
| S-EPMC4928274 | biostudies-literature
Unknown Generation of liver bipotential organoids with a small-molecule cocktail.
| S-EPMC7683013 | biostudies-literature
Unknown Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture.
| S-EPMC5425612 | biostudies-literature
Transcriptomics Long-term maintenance of functional primary human hepatocytes using small molecules
2019-09-01 | GSE135011 | GEO
Unknown mTOR supports long-term self-renewal and suppresses mesoderm and endoderm activities of human embryonic stem cells.
| S-EPMC2683106 | biostudies-literature
Unknown Cardiac induction of embryonic stem cells by a small molecule inhibitor of Wnt/?-catenin signaling.
| S-EPMC3076310 | biostudies-literature
Unknown Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells.
| S-EPMC2483414 | biostudies-other
Transcriptomics Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors
2011-04-28 | E-GEOD-28595 | biostudies-arrayexpress
Proteomics Small molecule inhibitor of NEDD4
2020-05-17 | MSV000085432 | MassIVE