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Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes.

ABSTRACT: Using phage display and IgG of a goat infected with Caprine Arthritis Encephalitis Virus (CAEV) we obtained families of 7 mer constrained peptides with consensus motifs LxSDPF/Y and SWN/KHWSY and mapped the epitopes mimicked by them at the Env 6-LISDPY-11 and 67-WNTYHW-72 sites of the mature gp135 amino acid sequence. The first epitope fell into the N-terminal immunogenic aa1-EDYTLISDPYGFS- aa14 site identified previously with a synthetic peptide approach; the second epitope has not been described previously. The first epitope is mostly conserved across CAEV isolates whereas the second newly described epitope is extremely conserved in Small Ruminant Lentiviruses env sequences. As being immunodominant, the epitopes are candidate targets for mimotope-mediated diagnosis and/or neutralization.

SUBMITTER: Gazarian K

PROVIDER: S-EPMC3162499 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes.

Gazarian Karlen K Setién Alvaro Aguilar AA Gazarian Tatiana T Pierle Sebastian Aguilar SA

Veterinary research 20110722

Using phage display and IgG of a goat infected with Caprine Arthritis Encephalitis Virus (CAEV) we obtained families of 7 mer constrained peptides with consensus motifs LxSDPF/Y and SWN/KHWSY and mapped the epitopes mimicked by them at the Env 6-LISDPY-11 and 67-WNTYHW-72 sites of the mature gp135 amino acid sequence. The first epitope fell into the N-terminal immunogenic aa1-EDYTLISDPYGFS- aa14 site identified previously with a synthetic peptide approach; the second epitope has not been describ ...[more]

PMID: 21781322

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Variability and immunogenicity of caprine arthritis-encephalitis virus surface glycoprotein.

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| S-EPMC112118 | biostudies-literature

Two species of Rev proteins, with distinct N termini, are expressed by caprine arthritis encephalitis virus.

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Host range of small-ruminant lentivirus cytopathic variants determined with a selectable caprine arthritis- encephalitis virus pseudotype system.

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| S-EPMC114973 | biostudies-literature

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