Ontology highlight
ABSTRACT:
SUBMITTER: Batchelder C
PROVIDER: S-EPMC316391 | biostudies-literature | 1999 Jan
REPOSITORIES: biostudies-literature
Batchelder C C Dunn M A MA Choy B B Suh Y Y Cassie C C Shim E Y EY Shin T H TH Mello C C Seydoux G G Blackwell T K TK
Genes & development 19990101 2
In the early Caenorhabditis elegans embryo, maternally expressed PIE-1 protein is required in germ-line blastomeres to inhibit somatic differentiation, maintain an absence of mRNA transcription, and block phosphorylation of the RNA polymerase II large subunit (Pol II) carboxy-terminal domain (CTD). We have determined that PIE-1 can function as a transcriptional repressor in cell culture assays. By fusing PIE-1 sequences to the yeast GAL4 DNA-binding domain, we have identified a PIE-1 repression ...[more]