Ontology highlight
ABSTRACT:
SUBMITTER: Ravassard P
PROVIDER: S-EPMC3163974 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Ravassard Philippe P Hazhouz Yasmine Y Pechberty Séverine S Bricout-Neveu Emilie E Armanet Mathieu M Czernichow Paul P Scharfmann Raphael R
The Journal of clinical investigation 20110825 9
Despite intense efforts over the past 30 years, human pancreatic β cell lines have not been available. Here, we describe a robust technology for producing a functional human β cell line using targeted oncogenesis in human fetal tissue. Human fetal pancreatic buds were transduced with a lentiviral vector that expressed SV40LT under the control of the insulin promoter. The transduced buds were then grafted into SCID mice so that they could develop into mature pancreatic tissue. Upon differentiatio ...[more]