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Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.

ABSTRACT: We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.

SUBMITTER: Chen CH

PROVIDER: S-EPMC3165005 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.

Chen Chia-Hung CH Sarkar Aniruddh A Song Yong-Ak YA Miller Miles A MA Kim Sung Jae SJ Griffith Linda G LG Lauffenburger Douglas A DA Han Jongyoon J

Journal of the American Chemical Society 20110620 27

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample vol ...[more]

PMID: 21671557

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