Ontology highlight
ABSTRACT:
SUBMITTER: Spurlock CF
PROVIDER: S-EPMC3165146 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Spurlock Charles F CF Aune Zachary T ZT Tossberg John T JT Collins Patrick L PL Aune Jessica P JP Huston Joseph W JW Crooke Philip S PS Crooke Philip S PS Olsen Nancy J NJ Aune Thomas M TM
Arthritis and rheumatism 20110901 9
<h4>Objective</h4>Low-dose methotrexate (MTX) is an effective therapy for rheumatoid arthritis (RA), yet its mechanism of action is incompletely understood. The aim of this study was to explore the induction of apoptosis by MTX.<h4>Methods</h4>Flow cytometry was performed to assess changes in the levels of intracellular proteins, reactive oxygen species (ROS), and apoptosis. Quantitative polymerase chain reaction was performed to assess changes in the transcript levels of select target genes in ...[more]