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Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of Listeria monocytogenes.

ABSTRACT: A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.

SUBMITTER: Lomonaco S

PROVIDER: S-EPMC3165415 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of Listeria monocytogenes.

Lomonaco Sara S Knabel Stephen J SJ Dalmasso Alessandra A Civera Tiziana T Bottero Maria Teresa MT

Applied and environmental microbiology 20110708 17

A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes. ...[more]

PMID: 21742911

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