Scaffoldin modules serving as "cargo" domains to promote the secretion of heterologous cellulosomal cellulases by Clostridium acetobutylicum.
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ABSTRACT:
SUBMITTER: Chanal A
PROVIDER: S-EPMC3165433 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
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Scaffoldin modules serving as "cargo" domains to promote the secretion of heterologous cellulosomal cellulases by Clostridium acetobutylicum.
Chanal Angélique A Mingardon Florence F Bauzan Marielle M Tardif Chantal C Fierobe Henri-Pierre HP
Applied and environmental microbiology 20110715 17
The secretion of large heterologous cellulases by Clostridium acetobutylicum was formerly shown to be deleterious. To circumvent this issue, various scaffoldins' modules were grafted at their N termini. Family 3a cellulose binding module combined with an X2 module(s) was found to trigger the secretion of Clostridium cellulolyticum cellulases by the solventogenic bacterium. ...[more]
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