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Real-time detection of blaKPC in clinical samples and surveillance specimens.


ABSTRACT: A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity.

SUBMITTER: Mangold KA 

PROVIDER: S-EPMC3165576 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Real-time detection of blaKPC in clinical samples and surveillance specimens.

Mangold Kathy A KA   Santiano Kristine K   Broekman Ronit R   Krafft Catherine A CA   Voss Barbara B   Wang Vivien V   Hacek Donna M DM   Usacheva Elena A EA   Thomson Richard B RB   Kaul Karen L KL   Peterson Lance R LR  

Journal of clinical microbiology 20110706 9


A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity. ...[more]

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