Protein stabilization in a highly knotted protein polymer.
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ABSTRACT:
SUBMITTER: Sayre TC
PROVIDER: S-EPMC3165941 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Publications
Protein stabilization in a highly knotted protein polymer.
Sayre Tobias C TC Lee Toni M TM King Neil P NP Yeates Todd O TO
Protein engineering, design & selection : PEDS 20110613 8
The polypeptide backbones of a few proteins are tied in a knot. The biophysical effects and potential biological roles of knots are not well understood. Here, we test the consequences of protein knotting by taking a monomeric protein, carbonic anhydrase II, whose native structure contains a shallow knot, and polymerizing it end-to-end to form a deeply and multiply knotted polymeric filament. Thermal stability experiments show that the polymer is stabilized against loss of structure and aggregati ...[more]
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