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Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.

ABSTRACT: MazF is an mRNA interferase which cleaves mRNAs at a specific sequence. Here, we show that in contrast to MazF-ec from Escherichia coli, which specifically cleaves ACA sequences, MazF-bs from Bacillus subtilis is an mRNA interferase that specifically cleaves a five-base sequence, UACAU. MazF homologues widely prevailing in Gram-positive bacteria were found to be highly homologous to MazF-bs, suggesting that they may also have similar cleavage specificity. This cleavage site is over-represented in the B. subtilis genes associated with biosynthesis of secondary metabolites, suggesting that MazF-bs may be involved in the regulation of the production of secondary metabolites.

SUBMITTER: Park JH

PROVIDER: S-EPMC3167231 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.

Park Jung-Ho JH Yamaguchi Yoshihiro Y Inouye Masayori M

FEBS letters 20110713 15

MazF is an mRNA interferase which cleaves mRNAs at a specific sequence. Here, we show that in contrast to MazF-ec from Escherichia coli, which specifically cleaves ACA sequences, MazF-bs from Bacillus subtilis is an mRNA interferase that specifically cleaves a five-base sequence, UACAU. MazF homologues widely prevailing in Gram-positive bacteria were found to be highly homologous to MazF-bs, suggesting that they may also have similar cleavage specificity. This cleavage site is over-represented i ...[more]

PMID: 21763692

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