Ontology highlight
ABSTRACT:
SUBMITTER: Koronakis V
PROVIDER: S-EPMC3167530 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Koronakis Vassilis V Hume Peter J PJ Humphreys Daniel D Liu Tao T Hørning Ole O Jensen Ole N ON McGhie Emma J EJ
Proceedings of the National Academy of Sciences of the United States of America 20110815 35
The WAVE regulatory complex (WRC) is a critical element in the control of actin polymerization at the eukaryotic cell membrane, but how WRC is activated remains uncertain. While Rho GTPase Rac1 can bind and activate WRC in vitro, this interaction is of low affinity, suggesting other factors may be important. By reconstituting WAVE-dependent actin assembly on membrane-coated beads in mammalian cell extracts, we found that Rac1 was not sufficient to engender bead motility, and we uncovered a key r ...[more]