Ontology highlight
ABSTRACT:
SUBMITTER: Roman-Hernandez G
PROVIDER: S-EPMC3168947 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Molecular cell 20110701 2
The ClpS adaptor delivers N-end rule substrates to ClpAP, an energy-dependent AAA+ protease, for degradation. How ClpS binds specific N-end residues is known in atomic detail and clarified here, but the delivery mechanism is poorly understood. We show that substrate binding is enhanced when ClpS binds hexameric ClpA. Reciprocally, N-end rule substrates increase ClpS affinity for ClpA(6). Enhanced binding requires the N-end residue and a peptide bond of the substrate, as well as multiple aspects ...[more]