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Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells.

ABSTRACT: Cells contain a large pool of nonpumping Na/K-ATPase that participates in signal transduction. Here, we show that the expression of ?1 Na/K-ATPase is significantly reduced in human prostate carcinoma as well as in several human cancer cell lines. This down-regulation impairs the ability of Na/K-ATPase to regulate Src-related signaling processes. A supplement of pNaKtide, a peptide derived from ?1 Na/K-ATPase, reduces the activities of Src and Src effectors. Consequently, these treatments stimulate apoptosis and inhibit growth in cultures of human cancer cells. Moreover, administration of pNaKtide inhibits angiogenesis and growth of tumor xenograft. Thus, the new findings demonstrate the in vivo effectiveness of pNaKtide and suggest that the defect in Na/K-ATPase-mediated signal transduction may be targeted for developing new anticancer therapeutics.

SUBMITTER: Li Z

PROVIDER: S-EPMC3173162 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells.

Li Zhichuan Z Zhang Zhongbing Z Xie Joe X JX Li Xin X Tian Jiang J Cai Ting T Cui Hongjuan H Ding Hanfei H Shapiro Joseph I JI Xie Zijian Z

The Journal of biological chemistry 20110722 37

Cells contain a large pool of nonpumping Na/K-ATPase that participates in signal transduction. Here, we show that the expression of α1 Na/K-ATPase is significantly reduced in human prostate carcinoma as well as in several human cancer cell lines. This down-regulation impairs the ability of Na/K-ATPase to regulate Src-related signaling processes. A supplement of pNaKtide, a peptide derived from α1 Na/K-ATPase, reduces the activities of Src and Src effectors. Consequently, these treatments stimula ...[more]

PMID: 21784855

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