Ontology highlight
ABSTRACT:
SUBMITTER: Stewart AP
PROVIDER: S-EPMC3173231 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
The Journal of biological chemistry 20110720 37
The epithelial sodium channel (ENaC) is a member of the ENaC/degenerin superfamily. ENaC is a heteromultimer containing three homologous subunits (α, β, and γ); however, the subunit stoichiometry is still controversial. Here, we addressed this issue using atomic force microscopy imaging of complexes between isolated ENaC and antibodies/Fab fragments directed against specific epitope tags on the α-, β- and γ-subunits. We show that for α-, β- and γ-ENaC alone, pairs of antibodies decorate the chan ...[more]