Ontology highlight
ABSTRACT:
SUBMITTER: Williams SL
PROVIDER: S-EPMC3175596 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Williams Siôn L SL Huang Jia J Edwards Yvonne J K YJ Ulloa Rick H RH Dillon Lloye M LM Prolla Tomas A TA Vance Jeffery M JM Moraes Carlos T CT Züchner Stephan S
Cell metabolism 20101201 6
Polg mtDNA mutator mice are important models for investigating the role of acquired mtDNA mutations in aging. Despite extensive study, there remains little consensus on either the etiology of the progeroid phenotype or the mtDNA mutation spectrum induced by disrupted polymerase-γ function. To investigate the latter, we have developed a novel, pragmatic approach we term "Mito-seq," applying next-generation sequencing to enriched, native mtDNA. Regardless of detection parameters we observed an inc ...[more]