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ABSTRACT:
SUBMITTER: Madden JT
PROVIDER: S-EPMC3176620 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Madden Jeremy T JT DeWalt Emma L EL Simpson Garth J GJ
Acta crystallographica. Section D, Biological crystallography 20110908 Pt 10
Two-photon excited ultraviolet fluorescence (TPE-UVF) microscopy is explored for sensitive protein-crystal detection as a complement to second-order nonlinear optical imaging of chiral crystals (SONICC). Like conventional ultraviolet fluorescence (UVF), TPE-UVF generates image contrast based on the intrinsic fluorescence of aromatic residues, generally producing higher fluorescence emission within crystals than the mother liquor by nature of the higher local protein concentration. However, TPE-U ...[more]