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In vivo imaging of the human retina using a two-photon excited fluorescence ophthalmoscope.

ABSTRACT: Noninvasive imaging of endogenous retinal fluorophores, including vitamin A derivatives, is vital to developing new treatments for retinal diseases. Here, we present a protocol for obtaining in vivo two-photon excited fluorescence images of the fundus in the human eye. We describe steps for laser characterization, system alignment, positioning human subjects, and data registration. We detail data processing and demonstrate analysis with example datasets. This technique allays safety concerns by allowing for the acquisition of informative images at low laser exposure. For complete details on the use and execution of this protocol, please refer to Bogusławski et al. (2022).¹.

SUBMITTER: Boguslawski J

PROVIDER: S-EPMC10140148 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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In vivo imaging of the human retina using a two-photon excited fluorescence ophthalmoscope.

Bogusławski Jakub J Tomczewski Sławomir S Dąbrowski Michał M Komar Katarzyna K Milkiewicz Jadwiga J Palczewska Grażyna G Palczewski Krzysztof K Wojtkowski Maciej M

STAR protocols 20230413 2

Noninvasive imaging of endogenous retinal fluorophores, including vitamin A derivatives, is vital to developing new treatments for retinal diseases. Here, we present a protocol for obtaining in vivo two-photon excited fluorescence images of the fundus in the human eye. We describe steps for laser characterization, system alignment, positioning human subjects, and data registration. We detail data processing and demonstrate analysis with example datasets. This technique allays safety concerns by ...[more]

PMID: 37058404

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