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High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells.

ABSTRACT: Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate.

SUBMITTER: Gutierrez E 

PROVIDER: S-EPMC3178539 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells.

Gutierrez Edgar E   Tkachenko Eugene E   Besser Achim A   Sundd Prithu P   Ley Klaus K   Danuser Gaudenz G   Ginsberg Mark H MH   Groisman Alex A  

PloS one 20110922 9

Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces  ...[more]

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