Ontology highlight
ABSTRACT:
SUBMITTER: Weber G
PROVIDER: S-EPMC3182018 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Weber Gert G Cristão Vanessa F VF de L Alves Flavia F Santos Karine F KF Holton Nicole N Rappsilber Juri J Beggs Jean D JD Wahl Markus C MC
Genes & development 20110715 15
Little is known about how particle-specific proteins are assembled on spliceosomal small nuclear ribonucleoproteins (snRNPs). Brr2p is a U5 snRNP-specific RNA helicase required for spliceosome catalytic activation and disassembly. In yeast, the Aar2 protein is part of a cytoplasmic precursor U5 snRNP that lacks Brr2p and is replaced by Brr2p in the nucleus. Here we show that Aar2p and Brr2p bind to different domains in the C-terminal region of Prp8p; Aar2p interacts with the RNaseH domain, where ...[more]