Ontology highlight
ABSTRACT:
SUBMITTER: Malinova A
PROVIDER: S-EPMC5461031 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Malinová Anna A Cvačková Zuzana Z Matějů Daniel D Hořejší Zuzana Z Abéza Claire C Vandermoere Franck F Bertrand Edouard E Bertrand Edouard E Staněk David D Verheggen Céline C
The Journal of cell biology 20170517 6
Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a central module of the spliceosome. Using quantitative proteomics, we identified assembly intermediates containing PRPF8, EFTUD2, and SNRNP200 in association with the HSP90/R2TP complex, its ZNHIT2 cofactor, and additional proteins. HSP90 and R2TP bind unass ...[more]