Ontology highlight
ABSTRACT:
SUBMITTER: Dua P
PROVIDER: S-EPMC3182346 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Dua Pooja P Yoo Jae Wook JW Kim Soyoun S Lee Dong-ki DK
Molecular therapy : the journal of the American Society of Gene Therapy 20110614 9
Off-target gene silencing is a major concern when using RNA interference. Imperfect pairing of the antisense strand with unintended mRNA targets is one of the main causes of small interfering RNA (siRNA) off-target silencing. To overcome this, we have developed "bulge-siRNA," a modified siRNA backbone structure with a single nucleotide (nt) bulge placed in the antisense strand. We found that siRNAs with a bulge at position 2 of the antisense strand were able to discriminate better between perfec ...[more]