Ontology highlight
ABSTRACT:
SUBMITTER: Liu H
PROVIDER: S-EPMC3182707 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Liu Hanqing H Chen Chunlai C Zhang Haibo H Kaur Jaskiran J Goldman Yale E YE Cooperman Barry S BS
Proceedings of the National Academy of Sciences of the United States of America 20110919 39
EF4 (LepA), a strongly conserved protein, is important for bacterial growth and functional protein biosynthesis under certain conditions and is quite similar structurally to the translocase EF-G. The elongation cycle in protein synthesis is characterized by ribosome oscillation between pretranslocation (PRE) and posttranslocation (POST) complexes. Here, using ensemble single turnover and equilibrium experiments, as well as single molecule FRET measurements, we demonstrate that EF4 can compete wi ...[more]