Ontology highlight
ABSTRACT:
SUBMITTER: Fraser JS
PROVIDER: S-EPMC3182744 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Fraser James S JS van den Bedem Henry H Samelson Avi J AJ Lang P Therese PT Holton James M JM Echols Nathaniel N Alber Tom T
Proceedings of the National Academy of Sciences of the United States of America 20110914 39
Modern protein crystal structures are based nearly exclusively on X-ray data collected at cryogenic temperatures (generally 100 K). The cooling process is thought to introduce little bias in the functional interpretation of structural results, because cryogenic temperatures minimally perturb the overall protein backbone fold. In contrast, here we show that flash cooling biases previously hidden structural ensembles in protein crystals. By analyzing available data for 30 different proteins using ...[more]