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Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions.

ABSTRACT: An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.

SUBMITTER: Wang X

PROVIDER: S-EPMC3182966 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions.

Wang Xiaoyu X Dong Ying Y Jiwani Ameena J AJ Zou Yonglong Y Pastor Johanne J Kuro-O Makoto M Habib Amyn A AA Ruan Minzi M Boothman David A DA Yang Chin-Rang CR

Proteome science 20110915

An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII ...[more]

PMID: 21917185

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