Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions.
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ABSTRACT: An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.
SUBMITTER: Wang X
PROVIDER: S-EPMC3182966 | biostudies-literature |
REPOSITORIES: biostudies-literature
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