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Stable-isotope labeling with amino acids in nematodes.

ABSTRACT: We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

SUBMITTER: Larance M

PROVIDER: S-EPMC3184259 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Stable-isotope labeling with amino acids in nematodes.

Larance Mark M Bailly Aymeric P AP Pourkarimi Ehsan E Hay Ronald T RT Buchanan Grant G Coulthurst Sarah S Xirodimas Dimitris P DP Gartner Anton A Lamond Angus I AI

Nature methods 20110828 10

We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms. ...[more]

PMID: 21874007

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