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Stable Isotope Labeling using Amino acids in Cell culture (SILAC) (mus.musculus)

ABSTRACT: Targeted SILAC determination of the rate of synthesis of proteins involved in mitochondrial structure and ETC activity in CT26 control and IF1-ablated cell lines

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Colon

SUBMITTER: Sonia Domínguez

LAB HEAD: José Manuel Cuezva

PROVIDER: PXD038108 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	060722_Sonia_KO_1.mgf	Mgf
	060722_Sonia_KO_1.mzid.gz	Mzid
	060722_Sonia_KO_1.raw	Raw
	060722_Sonia_KO_2.mgf	Mgf
	060722_Sonia_KO_2.mzid.gz	Mzid

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1 - 5 of 18

Publications

IF1 ablation prevents ATP synthase oligomerization, enhances mitochondrial ATP turnover and promotes an adenosine-mediated pro-inflammatory phenotype.

Domínguez-Zorita Sonia S Romero-Carramiñana Inés I Santacatterina Fulvio F Esparza-Moltó Pau B PB Simó Carolina C Del-Arco Araceli A Núñez de Arenas Cristina C Saiz Jorge J Barbas Coral C Cuezva José M JM

Cell death & disease 20230712 7

ATPase Inhibitory Factor 1 (IF1) regulates the activity of mitochondrial ATP synthase. The expression of IF1 in differentiated human and mouse cells is highly variable. In intestinal cells, the overexpression of IF1 protects against colon inflammation. Herein, we have developed a conditional IF1-knockout mouse model in intestinal epithelium to investigate the role of IF1 in mitochondrial function and tissue homeostasis. The results show that IF1-ablated mice have increased ATP synthase/hydrolase ...[more]

PMID: 37433784

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Stable Isotope Labeling using Amino acids in Cell culture (SILAC) (local data base)

Project description:Stable Isotope Labeling using Amino acids in Cell culture (SILAC) of CT26 control and IF1-ablated cells

2023-10-24 | PXD038109 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

Project description:Here we report the proteome content of exosomes released by epimastigote and bloodstream trypomastigotes forms of T. cruzi, the aetiological agent of Chagas disease. The exosomes were isolated using the ultracentrifugation method and further processed for LC-MS/MS. The results showed differential proteomic profiles of this two forms with marked differences in surface molecules cargoes. Noteworthy, the trans-sialidase (TS) surface proteins, a family of polymorphic virulence factors were differentially expressed between insect and human stages of T. cruzi highlighting the different surface nature of this two parasite stages.

2021-09-08 | PXD013876 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013878 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013880 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013882 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013901 | Pride

Trypanosoma cruzi PAN 4 exosomes LC-MS/MS

2021-09-08 | PXD013875 | Pride

Identification of proteins that interact with endogenous DHX9 from HeLa cell nuclear extracts

Project description:This project aims to identify proteins that interact with DHX9.

2024-06-12 | PXD044366 | Pride

Identification of muscle biomarkers in McArdle disease

Project description:Glycogen storage disease type V (GSDV, McArdle disease) is a rare genetic myopathy caused by deficiency of the muscle isoform of glycogen phosphorylase (PYGM). This results in a block in the use of muscle glycogen as an energetic substrate, with subsequent exercise intolerance. The GSDV pathophysiology is still not fully understood, especially with regard to some features such as muscle contractures or persistent muscle damage (i.e., even without prior exercise). We aimed at identifying muscle protein biomarkers of GSDV by analyzing the muscle proteome and the molecular networks associated with muscle dysfunction. Muscle biopsies from 8 patients and 8 controls were studied by quantitative protein expression using isobaric tags for relative and absolute quantitation (iTRAQ) followed by artificial neuronal networks (ANNs) and topology analysis. Protein candidate validation was performed by western-blot. Several proteins predominantly involved in the process of muscle contraction and/or calcium homeostasis, such as myosin, sarcoplasmic/endoplasmic reticulum calcium ATPase 1, tropomyosin alpha-1 chain, tro-ponin isoforms, and alpha-actinin-3 showed significantly lower expression levels in muscle of GSDV patients. These proteins could be potential biomarkers of the persistent muscle damage reported in GSDV patients. Further studies are needed to elucidate the molecular mechanisms by which PYGM controls the expression of these proteins.

2022-06-09 | PXD031605 | Pride

Cyclipostins and Cyclophostin analogs impair growth of Mycobacterium abscessus by inhibiting enzymes involved in many physiological processes

Project description:Mycobacterium abscessus is nowadays under the spotlight of the scientific community. This pathogenic mycobacteria is indeed responsible for a wide spectrum of infections involving mostly pulmonary infections in patients with cystic fibrosis. M. abscessus is intrinsically resistant to a broad range of antibiotics, including most antitubercular drugs, and is considered the most pathogenic and chemotherapy-resistant rapidly growing mycobacterium. Consequently, with very limited treatment options, the development of new therapeutic approaches to fight this pathogen are urgently needed. 38 new analogs of Cyclipostins & Cyclophostin (CyC), compounds naturally produced by Streptomyces species, have been synthesized. Their antibacterial activities against clinical isolates belonging to the M. chelonae-abscessus clade, as well as Gram-negative and Gram-positive bacteria have been evaluated by the REMA method. The intracellular activities of the CyC against intramacrophagic M. abscessus have also been investigated and compared to those of imipenem. The CyCs displayed very low toxicity towards host cells and their inhibitory activity was exclusively restricted to mycobacteria. The best candidate, CyC17, showed a high selectivity for mycobacteria with MIC values (<2 up to 40 µg/mL) comparable to those of most classical antibiotics used to treat M. abscessus infections. Of importance, several CyCs were active against extracellular M. abscessus growth (i.e., CyC17 / CyC18β / CyC25 / CyC26) or against intracellular mycobacteria inside macrophages (i.e., CyC7α,β / CyC8α,β) with MIC values similar to or better than those of standard antibiotics. Based on these results, we intended to identify the potential target enzymes of CyC17/CyC26 in M. abscessus by activity-based protein profiling (ABPP) approach coupled with mass spectrometry differential analysis.

2019-10-08 | PXD014255 | Pride

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