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Light-based feedback for controlling intracellular signaling dynamics.

ABSTRACT: The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.

SUBMITTER: Toettcher JE

PROVIDER: S-EPMC3184382 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Light-based feedback for controlling intracellular signaling dynamics.

Toettcher Jared E JE Gong Delquin D Lim Wendell A WA Weiner Orion D OD

Nature methods 20110911 10

The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability ...[more]

PMID: 21909100

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