Ontology highlight
ABSTRACT:
SUBMITTER: Bonnett SA
PROVIDER: S-EPMC3184853 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Bonnett Shilah A SA Rath Christopher M CM Shareef Abdur-Rafay AR Joels Joanna R JR Chemler Joseph A JA Håkansson Kristina K Reynolds Kevin K Sherman David H DH
Chemistry & biology 20110901 9
Polyketide natural products generated by type I modular polyketide synthases (PKSs) are vital components in our drug repertoire. To reprogram these biosynthetic assembly lines, we must first understand the steps that occur within the modular "black boxes." Herein, key steps of acyl-CoA extender unit selection are explored by in vitro biochemical analysis of the PikAIV PKS model system. Two complementary approaches are employed: a fluorescent-probe assay for steady-state kinetic analysis, and Fou ...[more]