Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.
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ABSTRACT:
SUBMITTER: Abraham S
PROVIDER: S-EPMC3187172 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Publications
Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.
Abraham Sam S Chin James J Brouwers Huub J M HJ Turner Bernadette B Zhang Ren R Chapman Toni A TA
Applied and environmental microbiology 20110729 18
Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8). ...[more]
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