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Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.

ABSTRACT: Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

SUBMITTER: Abraham S 

PROVIDER: S-EPMC3187172 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.

Abraham Sam S   Chin James J   Brouwers Huub J M HJ   Turner Bernadette B   Zhang Ren R   Chapman Toni A TA  

Applied and environmental microbiology 20110729 18

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8). ...[more]

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