Bioengineering anabolic vitamin D-25-hydroxylase activity into the human vitamin D catabolic enzyme, cytochrome P450 CYP24A1, by a V391L mutation.

ABSTRACT: CYP24A1 is a mitochondrial cytochrome P450 (CYP) that catabolizes 1?,25-dihydroxyvitamin D(3) (1?,25-(OH)(2)D(3)) to different products: calcitroic acid or 1?,25-(OH)(2)D(3)-26,23-lactone via multistep pathways commencing with C24 and C23 hydroxylation, respectively. Despite the ability of CYP24A1 to catabolize a wide range of 25-hydroxylated analogs including 25-hydroxyvitamin D(3), the enzyme is unable to metabolize the synthetic prodrug, 1?-hydroxyvitamin D(3) (1?-OH-D(3)), presumably because it lacks a C25-hydroxyl. In the current study we show that a single V391L amino acid substitution in the ?3a-strand of human CYP24A1 converts this enzyme from a catabolic 1?,25-(OH)(2)D(3)-24-hydroxylase into an anabolic 1?-OH-D(3)-25-hydroxylase, thereby forming the hormone, 1?,25-(OH)(2)D(3). Furthermore, because the mutant enzyme retains its basal ability to catabolize 1?,25-(OH)(2)D(3) via C24 hydroxylation, it can also make calcitroic acid. Previous work has shown that an A326G mutation is responsible for the regioselectivity differences observed between human (primarily C24-hydroxylating) and opossum (C23-hydroxylating) CYP24A1. When the V391L and A326G mutations were combined (V391L/A326G), the mutant enzyme continued to form 1?,25-(OH)(2)D(3) from 1?-OH-D(3), but this initial product was diverted via the C23 hydroxylation pathway into the 26,23-lactone. The relative position of Val-391 in the ?3a-strand of a homology model and the crystal structure of rat CYP24A1 is consistent with hydrophobic contact of Val-391 and the substrate side chain near C21. We interpret that the substrate specificity of V391L-modified human CYP24A1 toward 1?-OH-D(3) is enabled by an altered contact with the substrate side chain that optimally positions C25 of the 1?-OH-D(3) above the heme for hydroxylation.

SUBMITTER: Kaufmann M 

PROVIDER: S-EPMC3190681 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature