Unknown

Dataset Information

0

Crystal structure of plasminogen activator inhibitor-1 in an active conformation with normal thermodynamic stability.


ABSTRACT: The serpin plasminogen activator inhibitor-1 (PAI-1) is a crucial regulator in fibrinolysis and tissue remodeling. PAI-1 has been associated with several pathological conditions and is a validated prognostic marker in human cancers. However, structural information about the native inhibitory form of PAI-1 has been elusive because of its inherent conformational instability and rapid conversion to a latent, inactive structure. Here we report the crystal structure of PAI-1 W175F at 2.3 ? resolution as the first model of the metastable native molecule. Structural comparison with a quadruple mutant (14-1B) previously used as representative of the active state uncovered key differences. The most striking differences occur near the region that houses three of the four mutations in the 14-1B PAI-1 structure. Prominent changes are localized within a loop connecting ?-strand 3A with the F helix, in which a previously observed 3(10)-helix is absent in the new structure. Notably these structural changes are found near the binding site for the cofactor vitronectin. Because vitronectin is the only known physiological regulator of PAI-1 that slows down the latency conversion, the structure of this region is important. Furthermore, the previously identified chloride-binding site close to the F-helix is absent from the present structure and likely to be artifactual, because of its dependence on the 14-1B mutations. Instead we found a different chlorine-binding site that is likely to be present in wild type PAI-1 and that more satisfactorily accounts for the chlorine stabilizing effect on PAI-1.

SUBMITTER: Jensen JK 

PROVIDER: S-EPMC3191012 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Crystal structure of plasminogen activator inhibitor-1 in an active conformation with normal thermodynamic stability.

Jensen Jan K JK   Thompson Lawrence C LC   Bucci Joel C JC   Nissen Poul P   Gettins Peter G W PG   Peterson Cynthia B CB   Andreasen Peter A PA   Morth J Preben JP  

The Journal of biological chemistry 20110621 34


The serpin plasminogen activator inhibitor-1 (PAI-1) is a crucial regulator in fibrinolysis and tissue remodeling. PAI-1 has been associated with several pathological conditions and is a validated prognostic marker in human cancers. However, structural information about the native inhibitory form of PAI-1 has been elusive because of its inherent conformational instability and rapid conversion to a latent, inactive structure. Here we report the crystal structure of PAI-1 W175F at 2.3 Å resolution  ...[more]

Similar Datasets

| S-EPMC4214104 | biostudies-literature
| S-EPMC4646234 | biostudies-literature
| S-EPMC3870673 | biostudies-literature
| S-EPMC6418500 | biostudies-literature
| S-EPMC3044959 | biostudies-literature
| S-EPMC7810990 | biostudies-literature
| S-EPMC15197 | biostudies-literature
| S-EPMC3543036 | biostudies-literature
| S-EPMC4957703 | biostudies-literature
| S-EPMC5428408 | biostudies-literature