Ontology highlight
ABSTRACT:
SUBMITTER: West GM
PROVIDER: S-EPMC3196059 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
West Graham M GM Chien Ellen Y T EY Katritch Vsevolod V Gatchalian Jovylyn J Chalmers Michael J MJ Stevens Raymond C RC Griffin Patrick R PR
Structure (London, England : 1993) 20110901 10
Mechanism of G protein-coupled receptor (GPCR) activation and their modulation by functionally distinct ligands remains elusive. Using the technique of amide hydrogen/deuterium exchange coupled with mass spectrometry, we examined the ligand-induced changes in conformational states and stability within the beta-2-adrenergic receptor (β(2)AR). Differential HDX reveals ligand-specific alterations in the energy landscape of the receptor's conformational ensemble. The inverse agonists timolol and car ...[more]