Ontology highlight
ABSTRACT:
SUBMITTER: Kaiser SE
PROVIDER: S-EPMC3196335 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Kaiser Stephen E SE Riley Brigit E BE Shaler Thomas A TA Trevino R Sean RS Becker Christopher H CH Schulman Howard H Kopito Ron R RR
Nature methods 20110710 8
The protein ubiquitin is an important post-translational modifier that regulates a wide variety of biological processes. In cells, ubiquitin is apportioned among distinct pools, which include a variety of free and conjugated species. Although maintenance of a dynamic and complex equilibrium among ubiquitin pools is crucial for cell survival, the tools necessary to quantify each cellular ubiquitin pool have been limited. We have developed a quantitative mass spectrometry approach to measure cellu ...[more]