Ontology highlight
ABSTRACT:
SUBMITTER: Lan W
PROVIDER: S-EPMC3210782 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Lan Wenxian W Wang Zhonghua Z Yang Zhongzheng Z Zhu Jing J Ying Tianlei T Jiang Xianwang X Zhang Xu X Wu Houming H Liu Maili M Tan Xiangshi X Cao Chunyang C Huang Zhong-Xian ZX
PloS one 20111108 11
To convert cyt c into a peroxidase-like metalloenzyme, the P71H mutant was designed to introduce a distal histidine. Unexpectedly, its peroxidase activity was found even lower than that of the native, and that the axial ligation of heme iron was changed to His71/His18 in the oxidized state, while to Met80/His18 in the reduced state, characterized by UV-visible, circular dichroism, and resonance Raman spectroscopy. To further probe the functional importance of Pro71 in oxidation state dependent c ...[more]