ADP-Mg2+ bound to the ATP-grasp domain of ATP-citrate lyase.

ABSTRACT: Human ATP-citrate lyase (EC 2.3.3.8) is the cytoplasmic enzyme that catalyzes the production of acetyl-CoA from citrate, CoA and ATP. The amino-terminal portion of the enzyme, containing residues 1-817, was crystallized in the presence of tartrate, ATP and magnesium ions. The crystals diffracted to 2.3 Å resolution. The structure shows ADP-Mg(2+) bound to the domain that possesses the ATP-grasp fold. The structure demonstrates that this crystal form could be used to investigate the structures of complexes with inhibitors of ATP-citrate lyase that bind at either the citrate- or ATP-binding site.

SUBMITTER: Sun T 

PROVIDER: S-EPMC3212355 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature